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The cDNA sequence of human NAMPT was amplified by PCR from the pGex-6p-three-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, College of Missouri). The PCR products were digested and cloned right into a pET21a+ vector applying NdeI and XhoI restriction enzymes. Some extent mutation was launched by the quick modify web site-directed mutagenesis a